Journal: Stem Cell Research & Therapy

Article Title: Cholangiogenic potential of human deciduous pulp stem cell-converted hepatocyte-like cells

doi: 10.1186/s13287-020-02113-8

Figure Lengend Snippet: SHED-HepT reconstructs intrahepatic bile ducts in livers of recipient CCl 4 -treated mice. a–e In vitro hepatobiliary function was analyzed in SHED-Heps. Gene expression of uridine 5′-diphospho-glucuronosyltransferase 1A1 ( UGT1A1 ) by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Amount of direct bilirubin by colorimetric assay ( b ). Representative images of bile transport were detected by CLF staining. Nuclei were stained with DAPI ( c ). Gene expression of membrane metalloendopeptidase ( MME ), ATP-binding cassette transporter B1 ( ABCB1 ), ABCB11, and ABCC2 by RT-qPCR d . Representative images of the expression of MME, ABCB1, ABCB11, and ABCC2 were detected by immunohistochemical analysis ( e ). a–e hPHep, human primary hepatocyte. a, b, d n = 5 for all groups. *** P < 0.005. nd, no detection. The graph bars represent the means ± SEM. a, d Results are shown as a ratio to hPHeps (hPHep = 1). c, e Scale bars, 20 μm. f, g Representative histograms of the expression of cell surface markers for hepatocyte progenitors and mesenchymal stem cells in SHED-Heps ( f ) and whole liver cells isolated from recipient mice (WLC) ( g ) were detected by FCM assay. EPCAM: epithelial cell adhesion molecule; MME: membrane metalloendopeptidase; NCAM1: neural cell adhesion molecule; PROM1: promin 1; R-PE: R-phycoerythrin. f Red solid line, target antibody-stained histograms; black solid line, isotype-matched control-stained histograms. g Area filed with red, target antibody-stained histograms; solid line, isotype-matched control-stained histograms

Article Snippet: Intact SHED and human intrahepatic biliary epithelial cells (AXOL, Cambridge, UK) were used as controls.

Techniques: In Vitro, Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Colorimetric Assay, Staining, Membrane, Binding Assay, Expressing, Immunohistochemical staining, Isolation, Control

Journal: International Journal of Molecular Sciences

Article Title: Elevated Levels of Endocannabinoids in Chronic Hepatitis C May Modulate Cellular Immune Response and Hepatic Stellate Cell Activation

doi: 10.3390/ijms16047057

Figure Lengend Snippet: ECs affect inflammatory and fibrogenic hepatic cells activities. ( A ) Inflammatory cytokines mRNA and ( B ) CB1 and CB2 mRNA in human hepatocytes by 2.5 µM AEA and 20 µM 2-AG; ( C ) Fibrosis-related mRNA in hHSC and hHSC co-cultivated with PBMC from CHC patients by 2.5 µM AEA and 20 µM 2-AG. * p < 0.05 vs corresponding non-treated control.

Article Snippet: Primary human hepatic stellate cells (hHSC) and primary human hepatocytes (hHep) were obtained from ScienCell TM Research Laboratories (Carlsbad, CA, USA).

Techniques: Control